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macs ls columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macs ls columns
    Macs Ls Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+columns/MS+Columns/pmc13418531-514-38-41
    Average 97 stars, based on 2429 article reviews
    macs ls columns - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Single Cell:

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood–brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
    Article Snippet: Tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec) according to the manufacturer's protocol. .. Single-cell suspensions were incubated with CD11b MicroBeads (130-049-601, Miltenyi Biotec) for 15 min at 4 °C, followed by magnetic separation using MACS columns (130-042-401, Miltenyi Biotec). ..

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood-brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain.
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Incubation:

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood–brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
    Article Snippet: Tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec) according to the manufacturer's protocol. .. Single-cell suspensions were incubated with CD11b MicroBeads (130-049-601, Miltenyi Biotec) for 15 min at 4 °C, followed by magnetic separation using MACS columns (130-042-401, Miltenyi Biotec). ..

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood-brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain.
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Magnetic Cell Separation:

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood–brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
    Article Snippet: Tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec) according to the manufacturer's protocol. .. Single-cell suspensions were incubated with CD11b MicroBeads (130-049-601, Miltenyi Biotec) for 15 min at 4 °C, followed by magnetic separation using MACS columns (130-042-401, Miltenyi Biotec). ..

    Article Title: Rbfox2 selectively governs hematopoietic stem cell self-renewal by regulating proteostasis
    Article Snippet: .. For ex vivo incorporation, bone marrow c-Kit positive cells were enriched with MACS columns (Miltenyi, catalog no. 130-042-401). ..

    Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia
    Article Snippet: Fresh decidual tissues were rinsed, mechanically fragmented and incubated in RPMI 1640 supplemented with 1 mg/ml of collagenase IV (Sigma, Cat# C5138, USA) and 150 U/ml of DNase I (Roche, Cat# 11,284,932,001, Switzerland) at 37°C for 1 h. The digested suspension was passed through a nylon filter and subsequently separated by a layered Percoll gradient (60%, 40%, 20%) to obtain the decidua immune cells (DICs). .. Live CD45 + cells were then sorted by a MACS Separator (Miltenyi Germany) and two MACS Columns (Miltenyi, Cat# 130–042–401, Germany) after DICs were stained with a Dead Cell Removal Kit (Miltenyi, Cat# 130–090–101, Germany) and CD45 MicroBeads, human (Miltenyi, Cat# 130–045–801, Germany). ..

    Article Title: Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood-brain barrier dysfunction, metabolic reprogramming, and increased inflammation in the aging brain.
    Article Snippet: .. For endothelial cell enrichment, single-cell suspensions were incubated with CD31 microbeads (Miltenyi Biotec, #130–097–418, 10 μL per sample) for 15 min at 4 °C, washed, and applied to LS MACS columns (Miltenyi Biotec, #130–042–401) placed in a magnetic field. ..

    Article Title: Dual-system engineered bacteria and dendritic cells enable precise intratumoral IL-12 delivery and potent antitumor immunity.
    Article Snippet: .. Red blood cells were lysed using ammonium chloride buffer (R1010, Sorabio), and CD8+ T cells were isolated via magnetic separation using CD8 MicroBeads and MACS Columns (130-117-044, Miltenyi Biotec). .. Purified CD8+ T cells were activated overnight in complete RPMI 1640 medium supplemented with 150 U/mL recombinant IL-2 (CM39, NovoProtein), and 1 μg/mL plate-bound anti-CD3 and anti-CD28 antibodies (11453D, Gibco).

    Article Title: Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage-fibroadipogenic progenitors crosstalk.
    Article Snippet: .. Upon reaching a homogeneous cell suspension, FAPs were 228 magnetically sorted using MACS columns (Miltenyi LD separation columns, 130-042-901; Miltenyi MS 229 Separation columns, 130-042-201) and magnetic beads in three steps: first we selected the CD45- population 230 (Miltenyi, 130-052-301), then Sca1+ cells (Miltenyi, 130-106-641) and finally the PDGFRα+ (Miltenyi, 130- 231 101-502) population in order to sort FAPs as CD45-/Sca1+/PDGFRα+ cells. .. FAPs were then cultured in DMEM 232 High Glucose (Sigma-Aldrich, D5796) supplemented with 20% FBS (Gibco, 10270-106), L-glutamine and 233 1% Pen/Strep (Gibco, 15140-122).

    Article Title: Autophagy flux during human aging is sex- and cell type-specific, and is associated with physical fitness
    Article Snippet: PolyA library prep was performed by the Salk Institute for Biological Studies Next Generation Sequencing and Genomics Core using the Illumina NovaSeq 6000 SP to generate paired-end reads (50 bp) at an average depth of ∼50 million reads per sample. iNs directly converted from UNA+ fibroblasts from 8 young (23-35 years) and 5 older (64-76 years) SHOCK cohort donors were analyzed. .. After three weeks of conversion, iNs were detached using TrypLE and purified by magnetic-associated cell sorting (MACS) using PSA-NCAM antibody-associated beads (Miltenyi cat# 130-092-966) over MACS columns (Miltenyi cat# 130-042-201). ..

    Ex Vivo:

    Article Title: Rbfox2 selectively governs hematopoietic stem cell self-renewal by regulating proteostasis
    Article Snippet: .. For ex vivo incorporation, bone marrow c-Kit positive cells were enriched with MACS columns (Miltenyi, catalog no. 130-042-401). ..

    Staining:

    Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia
    Article Snippet: Fresh decidual tissues were rinsed, mechanically fragmented and incubated in RPMI 1640 supplemented with 1 mg/ml of collagenase IV (Sigma, Cat# C5138, USA) and 150 U/ml of DNase I (Roche, Cat# 11,284,932,001, Switzerland) at 37°C for 1 h. The digested suspension was passed through a nylon filter and subsequently separated by a layered Percoll gradient (60%, 40%, 20%) to obtain the decidua immune cells (DICs). .. Live CD45 + cells were then sorted by a MACS Separator (Miltenyi Germany) and two MACS Columns (Miltenyi, Cat# 130–042–401, Germany) after DICs were stained with a Dead Cell Removal Kit (Miltenyi, Cat# 130–090–101, Germany) and CD45 MicroBeads, human (Miltenyi, Cat# 130–045–801, Germany). ..

    Isolation:

    Article Title: Dual-system engineered bacteria and dendritic cells enable precise intratumoral IL-12 delivery and potent antitumor immunity.
    Article Snippet: .. Red blood cells were lysed using ammonium chloride buffer (R1010, Sorabio), and CD8+ T cells were isolated via magnetic separation using CD8 MicroBeads and MACS Columns (130-117-044, Miltenyi Biotec). .. Purified CD8+ T cells were activated overnight in complete RPMI 1640 medium supplemented with 150 U/mL recombinant IL-2 (CM39, NovoProtein), and 1 μg/mL plate-bound anti-CD3 and anti-CD28 antibodies (11453D, Gibco).

    Suspension:

    Article Title: Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage-fibroadipogenic progenitors crosstalk.
    Article Snippet: .. Upon reaching a homogeneous cell suspension, FAPs were 228 magnetically sorted using MACS columns (Miltenyi LD separation columns, 130-042-901; Miltenyi MS 229 Separation columns, 130-042-201) and magnetic beads in three steps: first we selected the CD45- population 230 (Miltenyi, 130-052-301), then Sca1+ cells (Miltenyi, 130-106-641) and finally the PDGFRα+ (Miltenyi, 130- 231 101-502) population in order to sort FAPs as CD45-/Sca1+/PDGFRα+ cells. .. FAPs were then cultured in DMEM 232 High Glucose (Sigma-Aldrich, D5796) supplemented with 20% FBS (Gibco, 10270-106), L-glutamine and 233 1% Pen/Strep (Gibco, 15140-122).

    Magnetic Beads:

    Article Title: Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage-fibroadipogenic progenitors crosstalk.
    Article Snippet: .. Upon reaching a homogeneous cell suspension, FAPs were 228 magnetically sorted using MACS columns (Miltenyi LD separation columns, 130-042-901; Miltenyi MS 229 Separation columns, 130-042-201) and magnetic beads in three steps: first we selected the CD45- population 230 (Miltenyi, 130-052-301), then Sca1+ cells (Miltenyi, 130-106-641) and finally the PDGFRα+ (Miltenyi, 130- 231 101-502) population in order to sort FAPs as CD45-/Sca1+/PDGFRα+ cells. .. FAPs were then cultured in DMEM 232 High Glucose (Sigma-Aldrich, D5796) supplemented with 20% FBS (Gibco, 10270-106), L-glutamine and 233 1% Pen/Strep (Gibco, 15140-122).

    Purification:

    Article Title: Autophagy flux during human aging is sex- and cell type-specific, and is associated with physical fitness
    Article Snippet: PolyA library prep was performed by the Salk Institute for Biological Studies Next Generation Sequencing and Genomics Core using the Illumina NovaSeq 6000 SP to generate paired-end reads (50 bp) at an average depth of ∼50 million reads per sample. iNs directly converted from UNA+ fibroblasts from 8 young (23-35 years) and 5 older (64-76 years) SHOCK cohort donors were analyzed. .. After three weeks of conversion, iNs were detached using TrypLE and purified by magnetic-associated cell sorting (MACS) using PSA-NCAM antibody-associated beads (Miltenyi cat# 130-092-966) over MACS columns (Miltenyi cat# 130-042-201). ..

    FACS:

    Article Title: Autophagy flux during human aging is sex- and cell type-specific, and is associated with physical fitness
    Article Snippet: PolyA library prep was performed by the Salk Institute for Biological Studies Next Generation Sequencing and Genomics Core using the Illumina NovaSeq 6000 SP to generate paired-end reads (50 bp) at an average depth of ∼50 million reads per sample. iNs directly converted from UNA+ fibroblasts from 8 young (23-35 years) and 5 older (64-76 years) SHOCK cohort donors were analyzed. .. After three weeks of conversion, iNs were detached using TrypLE and purified by magnetic-associated cell sorting (MACS) using PSA-NCAM antibody-associated beads (Miltenyi cat# 130-092-966) over MACS columns (Miltenyi cat# 130-042-201). ..



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